High Pressure Liquid Chromatography (HPLC) is a well-established technique for the quantification of viral particles. HPLC is the gold standard used to quantify viral particles in vaccine and therapeutic development. Liquid chromatography is used to purify biomolecules. Fast Protein Liquid Chromatography (FPLC) is used to isolate proteins.
When creating a vaccine, you need to attack the protein spikes on the SARS-CoV-2 virus. Proteins on the SARS-CoV-2 spikes have become the targets for creating vaccines and anti-viral monoclonal antibodies. Before analyzing the proteins of interest, they must be purified in solution. FPLC has been the gold standard for protein purifications for many years. Many prominent researchers create a multi-step purification protocol using FPLC resins and FPLC systems. ICON Scientific offers complete solutions, including a wide range of FPLC components that can be expertly configured to suit your research application.
As the battle against SARS-CoV-2 virus continues to grow and enter new stages, researchers are relying on liquid chromatography techniques to provide accurate, efficient methods for analyzing potential vaccines and therapeutics.
Liquid chromatography (LC) is a critically important tool in therapeutic research, forming the basis of clinical trials based on RNA purification for the novel therapeutic vaccines. HPLC has been instrumental in pioneering research into the composition, method of action and structure of SARS-CoV-2. High Performance Liquid Chromatography (HPLC) is critical for quality control and quality assurance for the bioactive ingredients used in vaccines as well as therapeutics and all aspects of pharmaceutical development.
Liquid chromatography is a vital technique in many industries including pharmaceutical, production, MRNA production and development, cannabis/hemp analysis, food and beverage industries, manufacturing, quality control and more.
Vaccines need to be purified and filtered at different stages. One of the most important purification steps involves separation techniques using chromatography columns. In liquid chromatography the dissolved liquid (mobile phase) carries the analyte of interest through the column which is packed with a fixed material (stationary phase). Different chemical properties of the molecules within the analyte determine the retention time for the components of interest.
Liquid chromatography (LC) is an essential technique used for the purification of biomolecules including RNA. SARS-CoV-2 is an RNA-based virus. RNA purification uses liquid chromatography (LC) because it is performed at both higher temperature and higher pressure than normal LC. Higher pressure and higher temperature stimulate the denaturation process to remove protein complexes. The combination of high pressure and temperature and a binary solvent gradient prevent RNA degradation, while providing an effective RNA isolation. Liquid chromatography instruments can isolate the targeted messenger RNA, the mRNA and therefore analyze the vaccines efficacy.
Preparative HPLC pumps are used in the manufacturing of vaccines. Liquid nanoparticle (LNP) production equipment is used for the development of mRNA-based vaccines, lipid nanoparticles (LNPs) have proven to be a suitable delivery form. Lipid nanoparticles protect and encapsulating the active ingredient allowing it to be transported to the target cells in the human body. SARS-CoV-2 vaccines are the first large-scale production vaccines using lipid nanoparticles and mRNA in vaccine history. Icon Scientific supplies custom solutions for both small and large-scale production of vaccine lipid nanoparticles.
SARS-CoV-2 researchers are constantly fighting a battle as they screen potential antiviral treatments and continue to perfect vaccine development.
During these unprecedented times ICON Scientific can help you configure and customize liquid chromatography (HPLC) systems to assist your research into the structure of SARS-CoV-2 and its method of action.
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