Online Degasser

Why is online degassing so important?

Online degassing is important when doing HPLC, FPLC, GPC, and uHPLC because degassing will remove dissolved gas therefore avoiding bubble formation. Degassing will improve quantitative analysis. Online degassing replaces helium sparging.

Outgassing is a term used to describe dissolved gases coming out of solution. This occurs in HPLC systems where rough surfaces produce nucleation sites for bubble formation. Bubbles entering the HPLC systems will present a false signal to the detector.

Outgassing and bubble formation create problems for HPLC, FPLC, GPC and uHPLC systems.

Low pressure mixing: systems are more prone to bubble formation. The solvents used for the mobile phase are mixed before the pump. Outgassing affects the volume of liquid after the valve and before the pump because the valves are proportioned based on time and cannot discern whether there are bubbles in the liquid or not. This results in an inaccurate gradient.

• With high pressure mixing each solvent has its own pump and mixing takes place after the pumps. This configuration is less prone to outgassing issues.
• Outgassing creates bubbles in the flow cell
• Outgassing disrupts the column bed in FPLC ruining the separation process
• Bubble formation can be the cause of many performance issues, both with low pressure and high-pressure mixing

Our online degasser modules use proprietary technology. A flat membrane is incorporated on both sides of the cassette to provide maximum surface area with minimal volume. We optimize the efficiency of the membrane by precisely controlling its thickness to maximize degassing efficiency. Vacuum degassing is optimized because the membrane has a high gas permeability, ensuring both efficient and continuous gas removal, regardless of the flow rates. This technology also enhances the lifetime of the degasser.  This innovative technique enables the highest degassing efficiency in the industry. Our breakthrough in technology hugely improves traditional Teflon tube degassers that are commonplace today.

We offer 2-8 channel modules that are available to handle analytical to semi-prep applications. All modules work with HPLC, UHPLC, FPLC, and GPC. Custom OEM configurations are available.

Outgassing occurs when gasses that have dissolved into the solvent or buffer cause bubbles in pumps and detectors. Therefore, good chromatographic separations require degassing the solvents or buffers.

While the solvent flows through the Teflon AF® membrane, the dissolved gases are removed from the solvent by means of a vacuum on the other side of the membrane. Typically 50% of dissolved gas must be removed from the solvent stream to avoid bubble formation. Online degassers are used at universities, research institutions, and laboratories in the following areas: biochemistry analysis, chemical analysis, pharmaceutical analysis, food and beverage analysis, environmental analysis, chiral analysis and protein purifications. Our degassers are engineered for superior performance and the highest degassing efficiency in the industry. We use Teflon AF® in all of our analytical and semi-prep degassers. The benefit of online degassing is that it is a continuous process as opposed to helium sparging which is typically done prior to the chromatographic run. After the solvents are helium sparged, gases are reintroduced into the solvent providing inconsistent degassing which degrades over time.